ok gotcha.
DosBox or similiar would be my first recommendation, but you already nixed those in your OP.
Next best option is this:
http://www.make-bootable-disks.com/u...drive-120.html
its directions for making a Dos 6.22 bootable USB drive. You could even copy the game itself to the usb drive and have it available to play on just about any computer!
Dos 6.22 is the last M$ version of "pure" Dos - version 7x and above were the underpinnings for W9x and above - seriously pared down, less functional. I would not use anything above 6.22 if your looking for real DOS.