That shouldn't matter.
Did you try a different port on the router (though it is probably not a bad port)? It may also be a configuration issue with the router itself (like for example, things are going out successfully, but being routed back to the first computer not the second). Also are you using a DMZ? I would also try putting the second computer in LAN 1 to see if it works that way. That will help you figure out where the problem is.
You also may want to try doing a hardware reset on the router, this sometimes fixes issues like that (but it will put the router back to the factory settings). The usual procedure is to hold down the reset button (usually recessed in the back of the router) for 30 seconds, unplug the router while still holding down the button, count another 30, release and plug back in.
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