I know you've tried switching off your antivirus, but are you running a firewall or other system monitoring software? If so, test with it all switched off too.
Otherwise, I'm not sure. I don't know ME well enough to understand how file access permissions are managed.
It may help if you post a step-by-step list of what error messages you get (the full accurate message) when you select your existing career and press the Launch SH3! button.
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