Yes, but in reality subs could not stop completely if it wanted to maintain depth control. And even if you are going less than 1 knot you can still mess up your speed measurement if you look too far to the side of your bow. This does not occur if the bow is (or stern) pointed towards the target and the periscope is at 0 (180) bearing.
NYGM simulates this very well (It gets irritating)